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KMID : 0363320110320010056
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Journal of Korean Oriental Internal Medicine
2011 Volume.32 No. 1 p.56 ~ p.67
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Inhibitory Effect of Mori Ramulus on Oxidative Stress Induced by High Glucose in LLC- Cells
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Jang Soo-Young
Shin Hyeon-Cheol
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Abstract
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Objectives: Recent etiological studies show that oxidative stress might play a major role in the diabetes and its complications. Mori Ramulus (MR) has been known to have antioxidative, anti-inflammatory and antidiabetic effects. The methanol extract of MR was tested for its effectiveness in LLC-PK1 cells exposed to high glucose.
Methods: The cytoprotective effect of MR was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The antioxidative effect was measured in terms of generation amount of by 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA), NO by 4,5-diaminofluorescein (DAF-2), by dihydrorhodamine 123 (DHR 123) in the high glucose -treated LLC- cells. Western blotting was performed using anti-AGE, anti-RAGE, anti-MAPKs(ERK1/2, JNK, p38), anti-PI3K, anti-Akt, and anti-NF-B (p50, p65) respectively.
Results: MR extract reduced cell death and inhibited the generation of , NO, in the high glucose-treated LLC- cells. MR inhibited the expression of AGE, RAGE, MAPKs, PI3K, and Akt by means of decreasing NF-B activation. MR also inhibited NF-B activation itself.
Conclusions : These results indicate MR has cytoprotective, antioxidative, and anti-inflammatory effects. Therefore it is suggested that MR might prevent and cure diabetes and its complications.
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KEYWORD
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Mori Ramulus, Oxidative stress, AGE, MAPK, NF-B
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